三黄连合剂通过NLRP3/ASC/Caspase-1通路对哮喘患儿气道炎症的机制研究
Mechanism of Sanhuanglian Mixture on Airway Inflammation in Children With Asthma Through NLRP3/ASC/Caspase-1 Pathway
张晓 1梁剑 1王文佳 1游楚明1
作者信息
- 1. 广东省第二人民医院儿科,广东 广州 510317
- 折叠
摘要
目的 探讨三黄连合剂通过NOD样受体蛋白 3(NOD-like receptor protein domain associated protein 3,NLRP3)/凋 亡 相 关 颗 粒 样 蛋 白(apoptosis-associated speck-like protein containing a CARD,ASC)/半胱氨酸天冬氨酸蛋白酶 1(cysteine aspartic acid specific protease-1,Caspase-1)通路对哮喘患儿气道炎症的作用及机制.方法 选取 2021 年 1 月—2023 年 10 月就诊的 90 例哮喘患儿,采用数字表法随机分为观察组与对照组,各 45 例.观察 2 组哮喘患儿不良反应、1 s用力呼气容积(forced expiratory volume in the first second,FEV1%)、最大呼气流量(peak expiratory flow,PEF)、用力肺活量(forced vital capacity,FVC)、白细胞介素-1β(interleukin-1β,IL-1β)及TNF-α(tumor necrosis factor-α,TNF-α)表达水平差异.结果 观察组不良反应发生率为 6.66%,对照组为11.11%,差异无统计学意义(P＞0.05).观察组总有效率为 95.56%,高于对照组的 80.00%(P＜0.05).治疗后FEV1%、PEF、FVC、哮喘控制测试表(asthma control test,ACT)评分高于对照组(P＜0.05).治疗后观察组血清IL-1β、IL-6、IL-8、IL-17、IL-18 水平低于对照组(P＜0.05).治疗后观察组患儿血清NLRP3、ASC、Caspase-1、IL-1β蛋白表达水平低于对照组(P＜0.05).结论 三黄连合剂治疗能减轻哮喘患儿气道炎症反应,降低炎症因子水平,改善肺功能,减轻病情,提高疗效,其可能通过NLRP3/ASC/Caspase-1 通路发挥作用.
Abstract
Objective To explore the effect and mechanism of Sanhuanglian mixture on airway inflammation in children with asthma through the NOD-like receptor protein domain associated protein 3/Apoptosis-associated speck-like protein containing a CARD/Cysteine aspartic acid specific protease-1 pathway.Methods From January 2021 to October 2023,a total of 90 children with asthma were randomly divided into observation group and a control group,with 45 casea in each group.The adverse reactions,forced expiratory volume in the first second(FEV1%),peak expiratory flow(PEF),forced vital capacity(FVC),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)of the two groups of children with asthma were observed.Results The incidence of adverse reactions in the observation group was 6.66%,while in the control group was 11.11%,with no statistically significant difference(P>0.05).The total effective rate of the observation group was 95.56%,which was higher than the 80.00%of the control group(P<0.05).After treatment,FEV1%,PEF,FVC,Asthma control test,the ACT score were higher than that of the control group(P<0.05).Post treatment observation group serum IL-1 β was lower than those of the control group(P<0.05).After treatment,the serum NLRP3,ASC,Caspase-1,and IL-1β were lower than those of the control group(P<0.05).Conclusion The treatment of Sanhuanglian mixture can alleviate airway inflammation in children with asthma,reduce levels of inflammatory factors,improve lung function,alleviate the condition,and enhance therapeutic efficacy.It may play a role through the NLRP3/ASC/Caspase-1 pathway.
关键词
三黄连合剂/NLRP3/ASC/Caspase-1通路/不良反应/哮喘/炎症因子/气道炎症Key words
sanhuanglian mixture/NLRP3/ASC/Caspase-1 pathway/adverse reactions/asthma/inflammatory factors/airway inflammation引用本文复制引用
基金项目
广东省中医药局科研立项项目(2021)(20211018)
出版年
2024
NETL
NSTL
万方数据