目的 探讨三黄连合剂通过NOD样受体蛋白 3(NOD-like receptor protein domain associated protein 3，NLRP3)/凋 亡 相 关 颗 粒 样 蛋 白(apoptosis-associated speck-like protein containing a CARD，ASC)/半胱氨酸天冬氨酸蛋白酶 1(cysteine aspartic acid specific protease-1，Caspase-1)通路对哮喘患儿气道炎症的作用及机制。方法 选取 2021 年 1 月—2023 年 10 月就诊的 90 例哮喘患儿，采用数字表法随机分为观察组与对照组，各 45 例。观察 2 组哮喘患儿不良反应、1 s用力呼气容积(forced expiratory volume in the first second，FEV1%)、最大呼气流量(peak expiratory flow，PEF)、用力肺活量(forced vital capacity，FVC)、白细胞介素-1β(interleukin-1β，IL-1β)及TNF-α(tumor necrosis factor-α，TNF-α)表达水平差异。结果 观察组不良反应发生率为 6。66%，对照组为11。11%，差异无统计学意义(P＞0。05)。观察组总有效率为 95。56%，高于对照组的 80。00%(P＜0。05)。治疗后FEV1%、PEF、FVC、哮喘控制测试表(asthma control test，ACT)评分高于对照组(P＜0。05)。治疗后观察组血清IL-1β、IL-6、IL-8、IL-17、IL-18 水平低于对照组(P＜0。05)。治疗后观察组患儿血清NLRP3、ASC、Caspase-1、IL-1β蛋白表达水平低于对照组(P＜0。05)。结论 三黄连合剂治疗能减轻哮喘患儿气道炎症反应，降低炎症因子水平，改善肺功能，减轻病情，提高疗效，其可能通过NLRP3/ASC/Caspase-1 通路发挥作用。
Mechanism of Sanhuanglian Mixture on Airway Inflammation in Children With Asthma Through NLRP3/ASC/Caspase-1 Pathway
Objective To explore the effect and mechanism of Sanhuanglian mixture on airway inflammation in children with asthma through the NOD-like receptor protein domain associated protein 3/Apoptosis-associated speck-like protein containing a CARD/Cysteine aspartic acid specific protease-1 pathway.Methods From January 2021 to October 2023,a total of 90 children with asthma were randomly divided into observation group and a control group,with 45 casea in each group.The adverse reactions,forced expiratory volume in the first second(FEV1%),peak expiratory flow(PEF),forced vital capacity(FVC),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)of the two groups of children with asthma were observed.Results The incidence of adverse reactions in the observation group was 6.66%,while in the control group was 11.11%,with no statistically significant difference(P>0.05).The total effective rate of the observation group was 95.56%,which was higher than the 80.00%of the control group(P<0.05).After treatment,FEV1%,PEF,FVC,Asthma control test,the ACT score were higher than that of the control group(P<0.05).Post treatment observation group serum IL-1 β was lower than those of the control group(P<0.05).After treatment,the serum NLRP3,ASC,Caspase-1,and IL-1β were lower than those of the control group(P<0.05).Conclusion The treatment of Sanhuanglian mixture can alleviate airway inflammation in children with asthma,reduce levels of inflammatory factors,improve lung function,alleviate the condition,and enhance therapeutic efficacy.It may play a role through the NLRP3/ASC/Caspase-1 pathway.
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