Abnormal Expression of IGFBP3 Gene and Prognosis of Hepatocellular Carcinoma
Objective To investigate the clinical significance of insulin-like growth factor binding protein 3(IGFBP3)in hepatocellular carcinoma and its mechanism of action in HepG2 hepatocellular carcinoma cells.Methods In this study,weighted correlation network analysis(WGCNA)and differential expression gene analysis were used,the functional enrichment of IGFBP3 and its relationship with the clinicopathological features of hepatocellular carcinoma were analyzed using the GSE54236 dataset in the cancer genome atlas(TCGA)and gene expression omnibus(GEO)databases.HepG2 cells with IGFPB3 overexpression were constructed by lentivirus transfection technique.The migration and proliferation capacity of HepG2 were determined by scratch healing assay and cell counting kit-8(CCK-8)proliferation assay.Results A total of 54 common differentially expressed genes were identified from TCGA and GSE54236.Gene ontology(GO)analysis showed significant enrichment in biological processes such as epithelial cell proliferation,acute inflammatory response,and complement activation,cellular components mainly included collagen and cytoplasmic vesicles,molecular functions involved carbohydrate and peptide binding.Kyoto encyclopedia of genes andgenomes(KEGG)analysis indicated pathways involved in neuroactive ligand-receptor interaction,among others.IGFBP3 was a central gene significantly correlated with overall survival in liver cancer,expressed at low levels in liver cancer tissues,and associated with tumor staging and lymph node metastasis.HepG2 cells overexpressing IGFBP3 exhibited enhanced migration and proliferation capabilities.Conclusion IGFBP3 is low expressed in hepatocellular carcinoma and is associated with overall survival of patients with hepatocellular carcinoma.Overexpression of IGFBP3 promotes migration and proliferation of HepG2 hepatoma cells.
hepatocellular carcinomaprognosisinsulin-like growth factor binding protein 3geneproliferatemigration
万方数据
维普
