Objective To explore the causes and solutions of inconsistent ABO positive and negative typing of automatic blood group analyzer.Methods A total of 25 000 voluntary blood donors clinical data in the department of transfusion,the Second Hospital of Longyan,Fujian Province from June 2019 to December 2020 were retrospective analysed.Autovue innova fully automatic blood type analyzer was used to detect voluntary blood donors with ABO blood type.Patients with inconsistent positive and negative blood types were accurately identified,and the causes were analyzed to develop corresponding strategies.Results Using a fully automated analyzer to detect 80 blood type specimens that do not match the positive and negative types,the main reasons were analyzed as laboratory reagents and instruments,human factors in specimen operation(36.25%),ABO subtypes(10.00%),high titer cold agglutinin(10.00%),and reduced antigen antibody levels in middle-aged and elderly people(8.75%).Among them,55 specimens were identified and corrected manually;However,factors such as allogeneic antigens,low immunoglobulin,and M protein still require further laboratory review and correction.Conclusion There are many reasons for the inconsistency of positive and negative typing of automatic blood group analyzer.The samples can be further tested by manual test tube method.If the positive and negative results are still inconsistent after manual identification,the samples should be sent to the laboratory for further determination to ensure the safety of patients during blood transfusion.
关键词
全自动血型分析仪/正反型不符/ABO血型/疑难血型/手工测量/操作规程
Key words
fully automatic blood type analyzer/mismatch between positive and negative types/ABO blood type/difficult blood type/manual measurement/operating procedures
维普
万方数据