PFGE Typing and Drug Resistance Analysis of Listeria Monocytogenes in Guizhou Province From 2022 to 2023
Objective To understand the serotypes,pulse-field gel electrophoresis(PFGE)molecular typing characteristics and drug resistance of Listeria monocytogenes in Guizhou Province from 2022 to 2023,with the aim of providing laboratory data support for the prevention and control of Listeria monocytogenes in Guizhou province and enhancing the capacity for early warning and surveillance.Methods A total of 1 220 diarrheal disease-related samples were collected from the central laboratory of Pathogenic Pathogens Network of Guizhou Province from January 2022 to December 2023.According to the execution standard of GB 4789.30-2016"National Food Safety Standard Food Microbiological Examination:Listeria Monocytogenes",the samples were isolated and cultured,identified by fluorescent polymerase chain reaction(PCR),multiplex PCR serotype typing,PFGE molecular typing and drug susceptibility test according to the"National Pathogen Identification Network Laboratory Monitoring Technical Manual(trial version 2022)".Results A total of 424 strains were isolated from 1 220 diarrheal disease-related samples,including 11 strains of Listeria monocytogenes,with a positive rate of 2.59%(11/424).The 11 strains of Listeria monocytogenes were divided into 6 serotypes,and the serotypes were 1/2a,1/2b,1/2c,3a,3b and 3c.The resistance rate of 11 strains of Listeria monocytogenes to cefoxitin(FOX)was as high as 100%,the mediator rate of 11 isolates was 72.72%to chloroamphenicol(CHL),and the remaining 12 isolates[erythromyci(ERY),trime-thoprim/sulfamethoxazole(T/SUL),gentamicin(GEN),imipenem(IPM),benzoxacillin(OXA),clindamycin(CLI),ciprofloxacine(CIP),datomycin(DAP),ampicillin(AMP),tetracycline(TET),vancomycin(VAN),chloramphenical(PEN)]were sensitive to all drugs.The 11 strains of Listeria monocytogenes were divided into 7 PFGE band types after digestion by AscⅠ enzyme,and the similarity was 40%to 100%.Among them,3 strains had a similarity of up to 100%.Conclusion A family cluster of listeriosis occurred in Guizhou province from 2022 to 2023,and the same pollution source existed in meat products in Zunyi city,and the pollution source persisted.Guizhou Province had a variety of potentially pathogenic Listeria monocytogenes,but serious drug resistance had not yet occurred.
Guizhou provinceListeria monocytogenesisolation cultureserotypingpulse-field gel electrophoresisdrug resistance
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