Effects of different extracts of Nanwuling Compound on sleep improvement in mice
Objective To investigate the efficacy of different extracts of Nanwuling Compound in improving sleep in mice.Methods One hundred and twenty male KM mice were randomized into a blank control group(BCG),an ethanol extract group(EEG),a water extract group(WEG),and a mixed extract group(HEG).Mice in each group were given corre-sponding doses of the drug by gavage,0.1 ml/10 g,once a day,for 30 consecutive days,except that BCG mice were given equal amount of distilled water by gavage.After the last administration,the mice were subjected to mouse spontaneous loco-motor activity and direct sleep experiment,prolonging the threshold dose of pentobarbital sodium sleep time experiment and subthreshold dose of pentobarbital sodium hypnosis experiment.The effects of the three extracts on the sleep status of mice were compared.Results The experimental results showed that no direct sleep occurred in any group after the last adminis-tration,and there was no significant difference in spontaneous locomotor activity between the groups(P＞0.05).There were significant differences in sleep latency and sleep time between the groups(P＜0.05).Compared with mice in the BCG group,mice in EEG and HEG groups had shorter sleep latency and longer sleep time(P＜0.05).And the effect on prolon-ging sleep time in HEG group was better than that of the WEG group(P＜0.05).The falling-asleep rates in mice in groups of BCG,WEG,EEG and HEG were 20％(2/10),30％(3/10),60％(6/10)and 80％(8/10),respectively.Compared with mice in the BCG group,the falling-asleep rate of mice in the HEG group significantly increased(P＜0.05).Conclu-sion The ethanol extract and mixed extract of Nanwuling Compound have an effect on improving the sleep status in mice,and the effect of the latter is better.
万方数据
