Content determination of hydroxy safflower yellow A in hongyao preparation by high performance liquid chromatography
Objective To establish a high performance liquid chromatography(HPLC)method for determining the content of hydroxy safflower yellow A(HSYA)in Hongyao preparation.Methods The determination was performed on ZORBAX SB-C18(4.6 mmx250 mm,5 μm)and the mobile phase was methanol-acetonitrile-0.7％ phosphoric acid solution(72:26:2)with the flow rate of 0.8 ml/min.The column temperature was 30 ℃,and the detection wavelength was 403nm.Re-sults HSYA had good linear relationship in the range of 0.012 5-2.491 4 μg(r=1.000 0).The average recovery of HSYA was 100.16％ and RSD was 1.58％ .Conclusion The method has the advantages of simple operation,strong speci-ficity and good repeatability.It is suitable for the quality control of HSYA in Hongyao preparation.
High performance liquid chromatographyHongyao preparationHydroxy safflower yellow AContent deter-mination
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