High-performance liquid chromatography for co-determination of cyperotundone and α-cyperone concentrations in Zikou pills
Objective To develop an high-performance liquid chromatography(HPLC)for co-determination of cyperotun-done and α-cyperone in Zikou pills.Methods We developed an HPLC using ZORBAX SB-C18(250 mmx4.6 mm,5 μm)as the chromatography column,methanol-water(at a ratio of 65:35)as the mobile phase,1.0 ml/min as the flow rate,30 ℃ as the column temperature,and 254 nm as the measurement wavelength.Results Cyperotundone and α-cyper-one had a good linear relationship with chromatographic peak area in the range of 0.108 8-1.088 0 μg and 0.040 4-0.403 6 μg,respectively,and the average recoveries were 102.1％ and 103.0％ ,respectively.Conclusion This method is easy operable and accurate,which can be used for quality control of Zikou pills.
NETL
NSTL
万方数据
