Simultaneous determination of six kinds of amanita peptide toxins in urine by ultra performance liquid chromatography-tandem mass spectrometry with magnetic solid-phase extraction
Objective To establish a method for the simultaneous determination of α-amanitin,β-amanitin,γ-amanitin,phallacidin,phalloidin and phallisacin in urine by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with magnetic solid-phase extraction.Methods Urine samples were extracted and purified by C18 func-tionalized magnetic nanoparticles(Fe3O4@SiO2@C18).Six amanita peptide toxins were determined by a mass spectrometer coupled with electrospray ionization(ESI)and multiple reaction monitoring(MRM)mode.Results The linear range of six amanita peptide toxins was 0.50-50.00 ng/ml,and all showed good linear relationship.The limits of detection were 0.05-0.15 μg/kg and the limits of quantification were 0.15-0.50 μg/kg.The average recoveries of the method ranged from 83.5％to 102.3％,and the relative standard deviation(RSD)ranged from 2.55％to 5.67％.Conclusion The method is rapid,simple and accurate for the determination of six kinds of amanita peptide toxins in urine.
Amanita peptide toxinsMagnetic solid-phase extractionUrineUltra performance liquid chromatography-tandem mass spectrometry
万方数据
