Uncertainty evaluation for content determination of fluorouracil in compound fluorouracil oral solution by HPLC
Objective To evaluate the uncertainty for content determination of fluorouracil in compound fluorouracil oral solution by HPLC.Methods With reference to JJF 1135-2005 Evaluation of measurement uncertainty in chemical analy-sis and JJF 1059.1-2012 Evaluation and expression of measurement uncertainty,the mathematical models to evaluate and analyze the source of uncertainty in the content determination of fluorouracil were established.The synthetic uncertainty was calculated and the extended uncertainty of the measurement results under 95％confidence interval was determined.Results The extended uncertainty for the content determination of fluorouracil in compound fluorouracil oral solution by this meth-od was 1.6％,k=2.Conclusion In this study,an uncertainty evaluation method for the content determination of fluorou-racil in compound fluorouracil oral solution by HPLC is established.It is clear that the source of uncertainty is mainly the weighing of reference substance and the dilution of test substance,which provide theoretical guidance for improving the ac-curacy and scientificity of the test.
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