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进口水产品中单增李斯特菌毒力基因调查

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目的 为了研究进口水产品中单增李斯特菌毒力基因的分布情况。方法 以来自宁波口岸进口水产品中的66株单增李斯特菌为研究对象,利用PCR技术对单增李斯特菌的9个毒力基因(prfA、iap、actA、plcB、plcA、mpl、inlB、Hly、inlA)进行检测。结果 66株单增李斯特菌中16株出现了基因缺失,其余50株具有全部毒力基因,其中prfA基因检出率84。8% (56/66),iap基因检出率93。9% (62/66),mpl基因检出率95。5% (63/66),inlB基因检出率98。5% (65/66),inlA基因检出率98。5%(65/66)。结论 进口水产品中单增李斯特菌几乎都具有这9种毒力基因,对食品安全具有潜在的威胁,需要加强监测。
Survey on virulence gene of Listeria monocytogenes from imported aquatic products
Objective To study the virulent genes prevalence of Listeria monocytogenes (LM) isolated from imported aquatic products.Methods Polymerase chain reaction was used to identify 9 virulence genes including prfA,iap,actA,plcB,plcA,mpl,inlB,Hly,inlA from 66 Listeria monocytogenes which were isolated from imported aquatic products of Ningbo.Results Among 66 Listeria monocytogene strains,16 isolates were found virulence genes deletion.The positive rates of prfA,iap,mpl,inlB and inlA were 84.8% (56/66),93.9% (62/66),95.5% (63/66),98.5% (65/66),98.5% (65/66) respectively.Conclusion Virulence genes are common in Listeria monocytogene isolates from imported aquatic products of Ningbo.It is required to strengthen the surveillance of Listeria monocytogene for the potential threat to food safety.

Listeria monocytogenesVirulence genesSurvey

张岳灿、于纪棉、张玉琳、戴淑君、林瑛、劳华均、王建峰

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宁波卫生职业技术学院,浙江宁波315100

宁波出入境检验检疫局,浙江宁波315012

单增李斯特菌 毒力基因 调查

国家质检总局科研项目

2010IK169

2013

中国卫生检验杂志
中华预防医学会

中国卫生检验杂志

CSTPCD北大核心
影响因子:0.771
ISSN:1004-8685
年,卷(期):2013.23(15)
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