Discussion on the extended-spectrum beta-lactamases-producing genotypes and antimicrobial resistance patterns of Salmonella strains isolated from children in Hangzhou
Objective To analyze the serotypes,antibiotic resistance,phenotypes and genotypes of extended-spectrum β-lactamases(ESBLs) of Salmonella enteritis isolated from the local district children with diarrhea,so as to provide basis for epidemiological studies and clinical medication in children with diarrhea.Methods 116 strains of Salmonella was conducted for serum typing,susceptibility test were performed by MIC method and K-B disk diffusion method,and extended-spectrum β-lactamases(ESBLs) genes were amplified by polymerase chain reaction(PCR).Results Salmonella typhimurium was the main serotype,accounting for 44％,followed by Salmonella enteritidis accounting for 18.10％.The resistant rate of Salmonella to ampicillin was the highest(71.13％),that to piperacillin/tazobactam was the lowest (0.94％).The detection rate of ESBLs was 16.38％.The differences on the resistance rates of ESBLs with different phenotypes to beta lactam antibiotics and its inhibitor complex(except piperacillin/tazobactam,imipenem) were statistically significant.There were 6 strains harbored blaCTX-M gene and 6 strains harbored blaTEM gene,2 strains harbored blaCTX-Mcombining with blaTEM,and blaSHV genotype was not detected.Conclusion Salmonella typhimurium and Salmonella enteritis are the main pathogenic bacteria causing children diarrhea,and the resistance to antibiotics is severe.The drug resistance of positive ESBLs strains are mainly mediated by blaCTX-M genotype mostly.
NETL
NSTL
万方数据
维普
