Simultaneous determination of 10 plant regulators in vegetables by high performance liquid chromatography
Objective To establish the high-performance chromatographic method for simultaneous determination of ten plant regulators including thidiazuron,forchlorfenuron,abscisic acid,6-glycosyl amino purine,N6-Isoprene adenine,6-Benzyl amino purine,indole-3-butyric acid,indole-3-acetic acid,α-naphthaleneacetic acid,methyl 1-naphthylacetate in vegetables.Methods The plant regulators residues in samples were extracted by acetonitrile,and the excess acetonitrile was removed by evaporation.The residues were then dissolved in dichloromethane.The solution was cleaned up with NH2 cartridge.The analytes were eluted by methanol,and then concentrated.Chromatographic analysis was carried out on Agilent C18 column by methanol/water(0.1％ HCOOH) in gradient program,using both DAD and FLD as detectors.The retention time was qualitative,and the standard curve external standard method was used to quantify the retention time.Results The detection limits of 10 regulators were within 1.0 μg/kg-8.0 μg/kg.When the concentrations were within 0.020 μg/ml-5.00 μg/ml,the correlation coefficient(r) was above 0.999.The recoveries were within 70％-104％,the RSD of precision was less than 4.0％.Conclusion The method is simple with good sensitivity and reproducibility.The practical application brings less detection cost and period.It is suitable for primary units to popularize.
High efficiency liquid chromatographyUV-fluorescence detection methodPlant regulatorsVegetables
NETL
NSTL
万方数据
维普
