4种自身抗体联合检测对1型糖尿病诊断的意义
Significance of combined determination with four autoantibodies in diagnosis of type 1 diabetes
董倩 1杨锡琴 2赵长胜 1张海涛 1张贺秋 2冯晓燕2
作者信息
- 1. 唐山市丰南区钱营中心卫生院,河北唐山063301
- 2. 军事医学科学院基础医学研究所,北京100850
- 折叠
摘要
目的 探讨联合检测谷氨酸脱羧酶抗体(GADA)、蛋白质酪氨酸磷酸酶抗体(IA-2A)、锌转运体8抗体(ZnT8A)和胰岛特异的葡萄糖-6-磷酸酶催化亚基相关蛋白抗体(IGRPA)对1型糖尿病患者诊断的意义.方法 采用酶联免疫吸附法(ELISA)对45例1型糖尿病患者、50例2型糖尿病患者及50例正常对照者血清进行GADA、IA-2A、ZnT8A及IGRPA自身抗体的检测.结果 IA-2A、GADA、ZnT8A和IGRPA在1型糖尿病患者中检出率分别为28.89％、53.33％、71.11％和77.78％.4种自身抗体的阳性率在1型糖尿病患者中高于2型糖尿病组及正常对照组,差异有统计学意义(P＜0.05).自身抗体联合检测敏感度均高于单项组,GADA/IA-2A/ZnT8A/IGRPA 4项联合检测敏感度及曲线下面积(AUC)均为最高,分别为95.56％和0.988.结论 GADA、IA-2A、ZnT8A和IGRPA抗体的联合检测可以显著提高1型糖尿病的检出率,对1型糖尿病的诊断具有重要意义.
Abstract
Objective To explore the significance of the combined determination of glutamic acid decarboxylase antibody(GA-DA),insulinoma-associated 2 molecule antibody(IA-2A) and Zinc transporter 8 antibody and Islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantibody (IGRPA) in the diagnosis of type 1 diabetes.Methods GADA,IA-2A,ZnT8A and IGRPA autoantibody in serum were conducted for detection by enzyme-linked immunosorbent assay (ELISA) in 45 patients with type 1 diabetes mellitus,50 type 2 diabetic patients and 50 normal controls.Results The detection rates of IA-2A,GADA,ZnT8A and IGRPA in patients with type 1 diabetes were 28.89％,53.33％,71.11％ and 77.78％.The positive rate of four autoantibodies in type 1 diabetes mellitus was significantly higher than those in type 2 diabetes group and control group,with the differences of statistical significance(P ＜0.05).The sensitivity of the combined autoantibodies detection was higher than that of the single detection.The sensitivity and the area under the curve of GADA/IA-2A/ZnT8A/1G-RPA were 95.56％ and 0.988,respectively.Conclusion The combined detection of GADA,IA-2A,ZnT8A and IGRPA can significantly improve the detection rate of type 1 diabetes mellitus,and has important significance for the diagnosis of type 1 diabetes mellitus.
关键词
1型糖尿病/谷氨酸脱羧酶抗体/蛋白质酪氨酸磷酸酶抗体/锌转运体8抗体/胰岛特异的葡萄糖-6-磷酸酶催化亚基相关蛋白抗体Key words
Type 1 diabetes mellitus/Glutamic acid decarboxylase antibody/Insulinoma-associated 2 molecule antibody/Zinc transporter 8 antibody/Islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantibody引用本文复制引用
基金项目
十二五国家高技术研究发展计划(863计划)课题(20-11AA02A113)
出版年
2018
NETL
NSTL
维普
万方数据