Application of gene chip technology in rapid identification of clinical Nontuberculosis mycobacteria infection
Objective This paper aims to explore the feasibility of gene chip technology in rapid identification of clinical sus-pected Nontuberculous mycobacterium(NTM)infection.Methods From 2019 to 2021,sputum and alveolar lavage fluid sam-ples(Acid fast staining positive,TB DNA test negative)of some clinically suspected patients with NTM infection from a Grade 3A hospital in Quanzhou City were collected.After simple pretreatment,the samples were directly identified and typed by gene chip method.After isolation and culture,the samples were identified by mass spectrometry(bicinchoninic acid)and compared by sequencing.Results A total of 74 clinical suspected NTM samples were collected and detected directly by gene chip meth-od.A total of 65 samples were identified as NTM(65/74,87.84％).After isolation and culture,36 samples were identified as NTM(36/74,48.65％)by mass spectrometry.16s rRNA sequencing showed that 68 of 74 samples were NTM(68/74,91.89％),and the remaining 6 samples(6/74,8.11％)were Mycobacterium tuberculosis.The sensitivity of gene chip method was 95.59％(65/68),the detection rate was significantly higher than that of mass spectrometry(P＜0.05),and the specifici-ty was 100％.Conclusion Gene chip technology can be used for rapid identification of common clinical isolates of NTM and has important guiding value for early and rapid diagnosis and treatment of NTM disease.
NETL
NSTL
万方数据
维普
