Effect of coxsackievirus A6 infection on Toll-like receptor expression in Human Jurkat T cells
Objective This paper aims to observe the infection characteristics of coxsackievirus A6(CA6)in human Jurkat T cells and detect the expression of Toll-like receptor(TLR)and cytokine secretion after infection,so as to explore the infection and pathogenic mechanism of CA6.Methods Human Jurkat T cells were selected to establish CA6 virus cell infection model,and the morphological changes of cells induced by virus infection were examined.The mRNA expression of TLR1-TLR10 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR),and the dynamic expression profile of TLRs in cells was further analyzed by Real-Time PCR.The secretion levels of interleukin-6(IL-6),interleukin-8(IL-8)and tumor necrosis factor α(TNF-α)in cell culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Results CA6 can replicate and proliferate in human Jurkat T cells and cause cytopathic effects.RT-PCR results suggested that CA6 infection caused up-regulation of TLR7 expression in human Jurkat T cells.Real-Time PCR results showed that after 6 h,12 h,24 h and 48 h infection,the relative levels of TLR7 mRNA in the infected group were 1.69± 0.47,3.53±1.17,4.46±0.69 and 2.76±0.39,respectively.The results of the control group were significantly different(t=-2.53,t=-3.74,t=-8.64,t=-7.75,P=0.112,P=0.02,P=0.001,P=0.001,respectively).The detec-tion result of cytokine suggested that IL-8 secretion level was up-regulated after CA6 infection,which was significantly higher than that of the control group,and the difference was statistically significant(t=-49.75,t=-91.10,P＜0.05).Conclu-sion CA6 infection up-regulates the expression of TLR7 in Jurkat T cells.The inflammatory response to CA6 may associate with part of TLR signal transduction pathway.
Coxsackievirus A6Toll-like receptorHuman Jurkat T cellsCytokines
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