Impacts of naringenin on proliferation and apoptosis of bladder cancer cells by regulating glycogen synthase kinase 3 β/β-Catenin/Snail1 signaling pathway
Objective This paper aims to study the impacts of naringenin on the proliferation and apoptosis of bladder cancer cells by regulating the glycogen synthase kinase 3β(GSK-3β)/β-Catenin/Snail1 signaling pathway.Methods CCK-8 method was applied to detect the survival rate of human bladder cancer T24 cells treated with naringenin at 0 μg/ml,10 μg/ml,20 µg/ml,40 μg/ml,60 μg/ml,80 µg/ml and screen the appropriate concentration of naringenin.T24 cells cultured in vitro were randomly grouped into control group and naringenin group,lithium chloride group group,naringenin+lithium chloride group;after treatment with naringenin and lithium chloride,the expression of GSK-3β/β-Catenin/Snail1 signal pathway pro-tein was detected by Western blot;MTT assay and cell colony forming assay were applied to detect cell proliferation in each group;cell apoptosis was detected by flow cytometry;the expression of proliferation-related protein Ki67 and apoptosis-related protein Bel-2 and Bax of T24 cells in each group were detected by immunofluorescence.Results Compared with the control group,the expression of GSK-3 β/p-GSK-3 β,β-Catenin and Snail1 proteins,survival rate,colony formation rate,Ki67 expression index,Bel-2/Bax,tumor weight and tumor volume of naringenin group decreased(P＜0.05),and the apop-tosis rate increased(P＜0.05).The expression of GSK-3β/p-GSK-3β,β-Catenin and Snail1 proteins,survival rate,colony formation rate,Ki67 expression index,Bel-2/Bax,tumor weight and tumor volume increased in lithium chloride group(P＜0.05);and the apoptosis rate decreased(P＜0.05).Compared with naringenin group,the expression of GSK-3β/p-GSK-3β,β-Catenin and Snail1 proteins,survival rate,colony formation rate,Ki67 expression index,Bel-2/Bax,tumor weight and tumor volume increased in naringenin+lithium chloride group(P＜0.05),and the apoptosis rate decreased(P＜0.05).Conclusion Naringenin can inhibit the proliferation and tumorigenicity of bladder cancer cells in nude mice and pro-mote their apoptosis by inhibiting GSK-3β/β-Catenin/Snail1 pathway.
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