Simultaneous determination of 10 flavonoid compounds in health foods by high performance liquid chromatography
Objective This paper aims to study the effect of extraction solvent and extraction method on the experimental results,and establish a method for the simultaneous determination of 10 flavonoid compounds including daidzin,daidzein,genistin,genistein,rutin,camphenylol,apigenin,pinocembrin,chrysin,galangal in health foods by high performance liquid chromatography(HPLC).Methods The target analytes in sample were ultrasonically extracted with 80％methanol-water at room temperature for 30 min.The extraction solution was centrifuged for 10 min at 8 000 r/min,and the supernatant was col-lected through 0.22 μm microporous filter membrane,then separated on an Agilent ZORBAX Eclipse XDB C18 column(250 mmx4.6 mm,5 µm)with methanol-0.1％formic acid water as mobile phase at the flow rate of 1.0 ml/min.The tem-perature of column was 40℃.The target analytes were detected with a photodiode array detector at the wavelengths of 250 nm,260 nm,270 nm,289 nm and conducted for quantitative analysis by external standard method and applied to the practical deter-mination.Results Good separation of the 10 flavonoid compounds was observed,with the linearity of 0.02 μg/ml-100 µg/ml,the correlated coefficient＞0.999,and the detection limits of 0.002 g/kg-0.020 g/kg.The average recoveries of the method were in the range of 85.9％-109.0％,with the relative standard deviations(RSDs)less than 6.0％.The stability of the method meets the detection requirements.Conclusion This method has the advantages of simple operation,good repeat-ability,high sensitivity,high rapidity and efficiency.This method can separate all the target analytes within 40 min and has good chromatographic resolutions,which can be applied to the simultaneous determination of 10 flavonoid compounds in health foods.
Health foodsFlavonoid compoundsHigh performance liquid chromatography
NETL
NSTL
万方数据
维普
