A study on the expression of EIF5A2 in urine of patients with bladder cancer
Objective This paper aims to study the expression of eukaryotic translation initiation factor 5A2(EIF5A2)in the urine of patients with bladder cancer,so as to explore its application value in the diagnosis of bladder cancer and its relationship with the pathological grade and invasive status of bladder cancer.Methods Real-time fluorescence quantitative PCR(RT-PCR)was used to detect the expression of EIF5A2 mRNA in the urine of bladder cancer patients(41 cases)and healthy sub-jects(33 cases)and analyze the difference in expression of EIF5A2 mRNA in urine of bladder cancer patients(n=41)and healthy subjects(n=33).The expression of EIF5A2 in urine of patients with bladder cancer was analyzed to investigate the difference between the high-grade(n=28)and low-grade(n=13)groups.The expression of EIF5A2 in invasive bladder cancer(n=20)and non-invasive bladder cancer(n=21)was also analyzed.Results Urinary EIF5A2 mRNA expression in patients with bladder cancer was higher than that in control group,and the difference was statistically significant(P＜0.05).There was no statistical significance on the difference in EIF5A2 mRNA expression between high-grade and low-grade bladder cancer groups(P＞0.05).There was no statistical significance on the difference in the expression between invasive and non-invasive bladder cancer groups(P＞0.05).Conclusion The expression of EIF5A2 mRNA is increased in urine of the patients with carcinoma of urinary bladder,which has definite reference value in the diagnosis of bladder cancer,suggesting that EIF5A2 may be involved in the occurrence and development of urinary bladder cancer.In addition,the expression of EIF5A2 is not related to the pathological grade and invasive status of bladder cancer.
NETL
NSTL
万方数据
维普
