Effect of high expression circRNA5430 on proliferation and apoptosis of lung adenocarcinoma cell line
Objective This paper aims to investigate the effect of circRNA5430 expression on the proliferation and apoptosis in lung adenocarcinoma cells(LAC).Methods The stable expression of NCI-H1975 cell line was constructed by lentivirus infection and puromycin drug screening.The expression of circRNA5430 was detected by qRT-PCR.Apoptosis was detected by Annexin V-APC and 7-AAD double staining.The proliferation of lung adenocarcinoma cells was detected by CCK-8 method.Results The NCI-H1975 cell line with stable expression of circRNA5430 was obtained after 14 days of screening with puromycin.The level of circRNA5430 in the experimental group was higher than that in the control group.Compared with the control cell line,the level of apoptosis in the experimental group increased,and the difference between the two groups was statistically significant(P＜0.001).Compared with the control group,the cell proliferation ability of the experimental group decreased at 24 h,48 h and 72 h(P＜0.05).Conclusion The overexpression of circRNA5430 inhibits the proliferation of lung adenocarcinoma cell lines and promotes the apoptosis of lung adenocarcinoma cell lines.
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