A study on toxin distribution and molecular epidemic cloning of Clostridium difficile
Objective This study aims to conduct toxin detection and homological analysis of Clostridium difficile(C.difficile)in feces samples from collected patients with diarrhea,so as to provide theoretical basis for the clinical treatment of C.difficile.Methods Feces of diarrhea patients from January to December 2022 were collected for the screening of C.difficile using cycloserine-cefoxitin-fructose agar(CCFA),identifiedby Merieh mass spectrometer,and homological analysis of collected strains was performed by using different mass spectrometry and second-generation sequencing technology.Results Fecal sam-ples were collected from 947 diarrhea patients and 69 strains of C.difficile were identified,including 19 virus-producing strains and 50 non-virus-producing strains.A total of 12 toxins were detected by second-generation sequencing,among which the detection rates of toxB and CD0873 were all 100.0％,the detection rates of toxA,cbpA and cdtA were all 89.5％(17/19),and the detection rate of cwp66 was 10.5％(2/19).A total of 9 clonotypes were detected,mainly ST2 type(42.1％,8/19),followed by ST3,ST54 and ST81 type(10.5％,2/19),and other clonotypes were scattered.Compared with the second-generation sequencing,the coincidence rate in the homological analysis of ST81 and ST54 was 100％.The coincidence rate of ST2 is slightly higher with Zhongyuan Huiji than that with Merieux and BD-Bruker mass spectrometers.Conclusion In Jiaxing area,the prevalent clone of Clostridium difficile is ST2.BioMerieux and Zhongyuan Huiji mass spec-trometry have advantages in the homological analysis of Clostridium difficile.
NETL
NSTL
万方数据
维普
