Expression and clinical significance of SHCBP1 mRNA in glioma
Objective This paper aims to investigate the expression of SHCBP1 mRNA in glioma and its clinical signifi-cance.Methods The expression of SHCBP1 mRNA in glioma tissue and normal brain tissue in TCGA database was con-ducted for comparison.K-M curves were used to analyze the difference in median survival time between groups with high and low expression of glioma SHCBP1 mRNA.Cox regression was used to analyze the prognostic risk factors of glioma,and the correlation between SHCBP1 and the degree of immune cell infiltration was analyzed.The glioma cell lines U87 were divided into negative control group,blank group and silence group according to whether SHCBP1 gene was silenced or not.The changes of cell proliferation and invasion between the three groups were compared.Results Compared with normal tissue,the expression of SHCBP1 mRNA in glioma tissue was increased(P＜0.05).Compared with the glioma group with low SHCBP1 mRNA expression,the median survival time was shorter in the group with high SHCBP1 mRNA expres-sion(P＜0.001).Age,WHO grade,IDH status,1p/19q status,histological type and SHCBP1 expression were all independent risk factors for the prognosis of glioma patients(P＜0.05).In glioma,SHCBP1 was positively correlated with Th2 cells and macrophages(r=0.845,r=0.414,P＜0.001),and negatively correlated with pDC cells(r=-0.363,P＜0.001).Compared with the blank group and the negative control group,the protein and mRNA levels of SHCBP1 in the silent group were decreased(P＜0.05).Conclusion SHCBP1 is highly expressed in glioma,and SHCBP1 may be involved in the malignant biological behavior of glioma.
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万方数据
维普
