Pathway expression of peripheral blood CD36/mTORC1 and analysis of cellular immune inflammatory factors in high-risk puerperae with incision infection after cesarean section
Objective This paper aims to analyze the pathway expression of peripheral blood cluster of differentiation 36(CD36)/mammalian target of rapamycin complex 1(mTORC1)and cellular immune inflammatory factors in high-risk puerpe-rae with incision infection after cesarean section.Methods A total of 107 high-risk puerperae undergoing cesarean section in the hospital were enrolled between June 2018 and June 2022.According to presence or absence of incision infection,they were divided into infection group(21 cases)and non-infection group(86 cases).The distributions of cultured bacteria from inci-sion secretions in infection group were analyzed.The risk factors of incision infection after cesarean section were analyzed by lo-gistic regression analysis.The levels of peripheral blood CD36/mTORC1 pathways related proteins[CD36,Toll-like receptor 4(TLR4),focal adhesion kinase(FAK),mTORC1]were detected by Western blot(WB).The levels of peripheral blood cellular immune inflammatory factors[interleukin-1(IL-1),interleukin-6(IL-6),interleukin-8(IL-8),tumor nec-rosis factor-α(TNF-α)]were detected by enzyme-linked immunosorbent assay(ELISA).The correlation between periph-eral blood CD36,mTORC1 and cellular immune inflammatory factors was analyzed by Spearman correlation analysis.Results The main pathogen of incision infection was Gram-negative bacteria(67.44％).Logistic regression analysis showed that operation time ≥60 min,usage types of antibiotics ≥3 and invasive operation ≥3 times were independent risk factors for incision infection after cesarean section(P＜0.01).The levels of peripheral blood CD36/mTORC1 pathways related proteins and immune inflammatory factors in infection group were higher than those in non-infection group(P＜0.05).The levels of peripheral blood IL-1,IL-6,IL-8 and TNF-α were positively correlated with the levels of CD36 and mTORC1 proteins(P＜0.001).Conclusion The expression of peripheral blood CD36/mTORC1 pathways increase in high-risk puerperae with incision infection after cesarean section,which are positively correlated with the levels of cellular immune inflammatory factors.
High-risk puerperaCesarean sectionIncision infectionCluster of differentiation 36Mammalian target of rapa-mycin complex 1Cellular immune inflammatory factor
NETL
NSTL
万方数据
维普
