Aspergillus niger is a kind of harmful pathogenic fungi that can easily cause the mildew of the feed,food, grain and oil. Compared with the physical and chemical methods, the Aspergillus niger antiblastic by using biologic antibacterial agent have the advantage of time long, non-resistance, safety and health. In this paper, two effective natural antibacterial agents, cinnamaldehyde and citral were studed. With the normal growth of Aspergillus niger as a control, the effect of the cinnamaldehyde and citral were studed by Oxford Cup and the gaseous diffusion methods. The results showed that regardless of the method of Oxford cup or gas diffusion, the formation of the diameter of inhibited halo treated with citral were significantly greater than that treated with cinnamaldehyde. At the same concentration, the effect of citral restraining the mycelium morphology and sporangia were significantly better than that of cinnamaldehyde. And the effect of antiAspergillus niger by the method of gas diffusion was more obvious than that by the method of Oxford cup.