首页|杭州地区发酵乳制品中乳酸菌的分离及耐药谱分析

杭州地区发酵乳制品中乳酸菌的分离及耐药谱分析

扫码查看
目的 对杭州地区市售发酵乳制品中添加的乳酸菌进行分离及耐药谱分析,旨在了解长期饮用添加乳酸菌的乳制品是否存在潜在生物安全问题.方法 选择市场上最常见的6种发酵乳制品,用稀释涂皿法进行乳酸菌的分离,纯化后初步鉴定;用纸片扩散法进行耐药谱分析.结果 共分离出16株乳酸菌,所有菌株均对氨曲南耐药,10株对阿米卡星耐药,6株对复方星诺明耐药,5株对左氧氟沙星耐药,5株对环丙沙星耐药,5株对妥布霉素耐药,2株对庆大霉素耐药,2株对万古霉素耐药,1株对头孢他啶耐药,1株对头孢吡肟耐药,1株对呋喃妥因耐药,1株对四环素耐药.而对青霉素G和头孢唑啉均敏感.结论 杭州地区市售的六种发酵乳制品中添加的乳酸菌种类及活菌数满足国家标准,分离的乳酸菌均存在耐药性,部分有多重耐药,仍需进一步对乳酸菌的耐药传播进行监测.
Isolation and identification of Lactobacillus from Fermented Dairy Products in Hangzhou
Objective To analyse the isolation and identification of Lactobacillus from Fermented Dairy Products in Hangzhou,and investigate the potential biosafety threat of these probiotics.Methods Six kinds of the most common Fermented Dairy Products were choosen from the market and the lactic acid bacteria were isolated.Antimicrobial susceptibility of 14 common antibiotics was tested with disc diffusion K-B methods after species-level identification.Results 16 Lactobacillus strains were isolated totally.Conclusion Most of the Fermented Dairy Products contain sufficient viable Lactobacillus as required by the national standard.All of the Lactobacillus separate from Fermented Dairy Products have antibiotic resistance.A small quantity of the Lactobacillus are resistant to the multdrug resistant.

Fermented dairy productsLactobacillusAntibiotic resistance

周芳美、刘传杰、谈千千、朱凯妮、程东庆

展开 >

浙江大学医学院附属第一医院,浙江杭州310003

浙江中医药大学生命科学学院,浙江杭州310053

发酵乳制品 乳酸菌 耐药性

2014

中国微生态学杂志
中华预防医学会 大连医科大学

中国微生态学杂志

CSTPCDCSCD
影响因子:1.115
ISSN:1005-376X
年,卷(期):2014.26(1)
  • 5