Regulation of intestinal flora and short-chain fatty acids in MPTP-induced Parkinson's mice by Jianpi Yishen Tongluo Recipe
Objective To observe the effects of Jianpi Yishen Tongluo Recipe(JYTR)on the compositions of intestinal flora and short-chain fatty acids(SCFAs)in mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Par-kinson's disease.Methods C57BL/6 mice were randomly divided into three groups:Control group(n=1 5),MPTP group(n=15)and MPTP+JYTR group(n=1 5).The movement was assessed by using pole climbing,suspension and beam experi-ments.Striatal tyrosine hydroxylase(TH)expression was detected with immunofluorescence.Protein blotting was used to detect α-synuclein(α-syn)expression in striatum and colon.16S ribosomal RNA gene sequencing was used to assess the changes in the gut microbiota.Gas chromatography-mass spectrometry was used to analyze the content of SCFAs in mouse feces.Finally,correlation analysis was performed on the differential flora and SCFAs.Results Compared to the MPTP-treated group,mice in the MPTP+JYTR group exhibited an elevation in tail suspension test scores(P=0.040),alongside a pronounced decrease in both pole-climbing(P=0.042)and beam-walking times(P＜0.001).There was also a significant uptick in striatal TH expression(P=0.045),coupled with a notable dip in α-syn levels in both the striatum and colon(P=0.011,P=0.037).In terms of gut microbiota,there was a noteworthy augmentation in both alpha and beta diversity;phylum-level analyses revealed a surge in Bacteroidota abundance(P=0.003),whereas populations of Firmicutes and Ver-rucomicrobiota saw a significant downturn(P=0.001,P=0.011).At the family level,there was a significant influx in Lach-nospiraceae(P=0.001),while the relative abundance of Akkermansiaceae and Erysipelotrichaceae diminished substantially(P=0.008,P=0.004).Additionally,concentrations of the SCFAs acetate and propionate were significantly elevated(P=0.014,P=0.010).Correlation analyses suggested that the improvements in pole climbing and tail suspension tests,as well as beam walking times,were associated with shifts in the gut microbiota composition and SCFAs profiles.Conclusion JYTR may play a role in the treatment of Parkinson's disease by regulating the dysbiosis of intestinal flora and improving the levels of SCFAs.
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