Effects of viable triple Bifidobacterium capsule on intestinal barrier function and immune function in patients with chronic posthepatitis B cirrhosis
Objective To observe the effects of viable triple Bifidobacterium capsule on intestinal barrier function and immune function in patients with posthepatitis B cirrhosis based on the characteristics of intestinal flora.Methods A total of 124 patients with hepatitis B cirrhosis who received treatment in our hospital from January 2019 to December 2022 were enrolled and randomly divided into two groups with 62 patients in each group.The control group received conventional symptomatic treatment and intravenous infusion of magnesium isoglycyrrhizinate.The observation group was additionally treated with viable triple Bifidobacterium capsule on the basis of the control group.The total bilirubin(TBIL),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)were measured before and after treatment.Before and after treatment,peripheral venous blood was collected for detection of natural killer cells,CD3 and CD4 cells by using FAC-Scan flow cytometry.Feces of patients were collected before and after treatment,and the contents of Enterococcus,Bi-fidobacterium,Lactobacillus,Enterobacter and Bacteroides were detected using fluorescence quantitative polymerase chain valance.High performance liquid chromatography was used to record the lactulose/mannitol exclusion ratio before and after treatment.The content of IL-9 was detected with enzyme-related immunosorbent assay and Th9 cell was detected with flow cytometry.Adverse reactions during treatment were recorded.Results The levels of TBIL,AST and ALT in the observation group were lower than in the control group(all P＜0.05).The expressions of CD3,CD4 and NK cells in both groups increased(all P＜0.05),with those in observation group being higher than those in control group respectively(all P＜0.05).After treatment,compared with control group,the contents of Enterococcus and Enterobacter in observation group were lower,while those of Bacteroides,Lactobacillus and Bifidobacterium were higher(all P＜0.05).After treat-ment,the urine lactulose/mannitol exclusion ratio decreased in both groups,with that in the observation group being lower(P＜0.05).The expressions of IL-9 and Th9 cells in both groups decreased(P＜0.05),and the expressions of IL-9 and Th9 cells in observation group were lower than those in control group(P＜0.05).There was no adverse drug reaction in the two groups during treatment.Conclusion Viable triple Bifidobacterium capsule can improve intestinal flora,liver function,intestinal barrier function and immune function of patients with posthepatitis B cirrhosis.
Intestinal floraBifidobacterium triadCirrhosis of the liverIntestinal barrier functionImmune function
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