Effect of Akkermansia muciniphila on organ damage following high-temperature exercise in rats
Objective To observe the effect of Akkermansia muciniphila(A.muciniphila)on organ damage by interven-ing intestinal microbiota following high-temperature exercise in rats.Methods Sprague Dawley rats aged 6-8 weeks were randomly divided into control group(Control,n=5),room temperature training group(RT group,n=8),hyperthermia training group(HT group,n=8),A.muciniphila intervention group(AKK intervention group,n=8),recipients of feces from HT group(HT.R group,n=4)and recipients of feces from AKK intervention group(AKK.R group,n=4).Blood alanine aminotransferase(ALT),aspartate transaminase(AST),urea nitrogen(BUN)and creatinine(Cr)were measured.16S rRNA gene sequencing and GC-MS were used to observe gut microbiota structure and metabolite changes.Histological ex-aminations were performed on colon and liver tissues,and immunohistochemical staining was used to evaluate intestinal barrier function.Results The mean exercise durations of rats in the HT group and AKK intervention group were(13.76±3.454)minutes and(19.55±1.997)minutes,respectively,under conditions of(35±2)℃ and(55±5)％humidity,with statistically significant difference(t=4.104,P=0.001 1).After the intervention of A.muciniphila,the levels of AST(U/mL),ALT(U/mL),BUN(mmol/L)and Cr(pmol/L)in serum of rats(3.953±1.877,2.622±1.086,10.040±1.088,65.800±12.010)were lower than those in HT group(6.358±1.436,4.335±1.304,15.010±1.913,93.980±39.110),and the differences in the AST,ALT and BUN were statistically significant(t=2.878,t=2.856,t=-6.381,P=0.012 2,P=0.012 7,P＜0.000 1).The composition and structure of gut microbiota changed at the genus level,and levels of metabolites in feces showed varying degrees of changes.Histological examination results showed no significant damage to colon tissue,and liver damage was significantly alleviated compared to the HT group.Expression of intestinal junction proteins ZO-1 and Occludin was upregulated in the AKK intervention group(6 330±481.8,6 140±281.7)compared to the HT group(3 425±257.0、3 943±242.1),indicating a positive effect on the intestinal barrier(t=9.216,t=10.250,P=0.000 8,P=0.000 5).Conclusion Probiotics A.muciniphila can improve gut microbiota and alleviate colon and liver damage caused by high-temperature exercise by enhancing gut barrier function to a certain extent.
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