Correlation between microecology and antibiotic resistance at the pig-soil interface in Chongming District,Shanghai,China
Objective To observe microbial composition and antibiotic resistance genes(ARGs)in pig gut and soil flora and explore the correlation between them.Methods This research was conducted in five swine farms in Chongming Dis-trict,Shanghai.Samples of swine feces and soil were collected and subjected to Whole Genome Shotgun sequencing on the Illumina NovaSeq 6000 high-throughput sequencing platform.Through bioinformatics analysis and statistical testing,the study examined the association between microbial composition and ARGs.Results Metagenomic analysis revealed that the dominant phyla in swine manure in Chongming were Bacteroidetes,Firmicutes,Proteobacteria and Actinobacteria.Pre-valent types of ARGs included multidrug,tetracycline,glycopeptide,peptide,fluoroquinolone and beta-lactam,etc.Samples from the same pig farm exhibited correlations in terms of microbial abundance and ARGs diversity.Operational factors within farms,such as feed composition,stocking density,and hygiene conditions,lead to differences in microbial composition and ARGs.At the pig feces-soil interface,beneficial bacterial taxa exhibited symbiotic relationships.These bacteria could suppress the growth of pathogenic bacteria and also had an impact on soil microecology.Various types of ARGs were found to have symbiotic relationships among themselves.Conclusion This study investigates the composi-tion and differences in microecology and ARGs in pig feces and soil.It reveals the close association between microbiota and ARGs,proposing that the microbiota is a significant driver of ARG profiles.This finding provides a strong basis for antibiotic resistance prevention and control through microecological regulation.
Gut microbiotaSoil microbiotaMicrobial diversityAntibiotic resistanceMetagenomics
NETL
NSTL
万方数据
