The effect of lactic acid bacteria combined with Raelynn on HeLa cell activity and HPV18 viral load
Objective To observe the effects of lactic acid bacteria(LAB)fermentation broth(FB)and Raelynn on prolif-eration,apoptosis,migration and HPV18 DNA load of cervical cancer cell line HeLa.Methods The effects of different concentrations of single and combination drugs on the proliferation of HeLa cells were detected with CCK8 method,and the IC50 of LAB FB combined with Raelynn on HeLa cells was obtained.The effects of LAB FB and Raelynn on HeLa cell apoptosis,cell migration and HPV18 DNA viral load were examined using Flow cytometry,Transwell assay and hybrid capture-chemiluminescent assay(DH3).Results The IC50 of LAB FB was 5.5× 108 CFU/mL,and that of Raelynn was 160 mg/mL by CCK-8 method.The results of proliferation experiment showed that HeLa cells were inhibited significantly by single drug after 48 hours,and the effect in LAB FB+Raelynn group was stronger than that in Raelynn group(F=164.810,P＜0.05).The apoptosis of HeLa cells in LAB FB+Raelynn group was more than that in Raelynn group(F=83.823,P＜0.05).Transwell assay showed that cell migration in LAB FB+Raelynn group was inhibited more than that in Raelynn group(F=492.242,P＜0.05),and DH3 method showed that HPV18 DNA load of HeLa cells was down-regulated in both groups,the effect of LAB FB+Raelynn group on the viral load of HPV18 DNA was greater than that in Raelynn group(F=284.769,P＜0.05).Conclusion The effect of LAB FB combined with Raelynn for inhibiting proliferation,inducing apoptosis,inhibiting cell migration and down-regulating HPV18 DNA viral load of HeLa cells is superior to that of Raelynn,which may provide experimental basis and reference for clinical treatment of HPV infection.
Lactic acid bacteriaRaelynnCell proliferationCell apoptosisHPV18 DNA viral load
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