Intestinal microbiome and non-target metabonomics indicators in patients with Crohn's disease after amino acid-based enteral nutrition support
Objective To observe the changes of non-target metabonomics indicators and intestinal flora in patients with Crohn's disease(CD)supported with amino acid-based enteral nutrition preparation,providing a reference for the treat-ment.Methods Twenty patients with CD during the active phase treated in our hospital were selected as the subjects.All the patients were treated with amino acid-type enteral nutrition preparation.The differences in the structure and diversity of intestinal flora and non-target metabolomics before and after treatment were compared.Results After treatment,the in-testinal Lactobacillus(t=5.200,P＜0.001),Escherichia coli(t=11.974,P＜0.001),Klebsiella(t=15.033,P＜0.001),Sac-charomonospora(t=12.166,P＜0.001),Pseudomonas(t=31.063,P＜0.001),Enterococci(t=28.867,P＜0.001),intestinal flora OUTs(t=40.435,P＜0.001),Observed species(t=5.475,P＜0.001),Chao1 index(t=12.348,P＜0.001),Simpson in-dex(t=2.961,P=0.005)and Shannon index(t=3.330,P=0.002)significantly increased;the levels of amino acids,poly-peptides,fatty acids,cholesterol and carbohydrates in the serum and feces of patients were significantly improved com-pared with those before treatment.Conclusion After the support of amino acid based enteral nutrition,the abundance of microbial communities in patients with CD increases,and the improvement of lipid metabolism may be associated with the pathways related to oxidative stress response.
NETL
NSTL
万方数据
