Regulatory effect of fructooligosaccharide on helper T cells in AOM/DSS induced colitis-associated colorectal cancer mice
Objective To observe the effect of prebiotic fructooligosaccharide(FOS)on intestinal helper T cells in colitis-associated colorectal cancer(CAC)mice.Methods Six-week-old SPF male C57BL6 mice were randomly divided into the control group(Control group),model group(AOM/DSS group),and intervention group(AOM/DSS-FOS group).The CAC model was established by using azoxymethane and dextran sulfate sodium.The intervention group was given 2 mg/kg b.w.FOS by gavage for 10 weeks.The weight change in each group was monitored during the modeling period,and the colon length and tumor number were measured after the modeling.Serum Th1/Th2/Th17-related cytokines were detected with Cytometric Beads Array,and the proportions of Th1,Th2 and Th17 cells in mesenteric lymph nodes were analysed by flow cytometry.Results Compared with the AOM/DSS group,the colon length(t=3.106,P=0.006 4)increased,the num-ber of tumors(U=15.000,P=0.0111)decreased,the serum levels of proinflammatory cytokines such as IL-17A(t=3.504,P=0.008 8)and TNF-α(t=2.381,P=0.030 0)significantly decreased,the proportion of Th17 cell(t=6.031,P=0.0027)in mesenteric lymph nodes significantly decreased,and Th1/Th2(t=2.419,P=0.038 7)significantly increased in the interven-tion group.Conclusion Fructooligosaccharide can regulate the differentiation of helper T cells in the colon of CAC mod-el mice,and inhibit the occurrence of tumors.
Colorectal cancerFructooligosaccharideHelper T cellMucosal immune
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