Effect of Shou-Hui Tongbian Capsules on intestinal function in mice with postoperative ileus
Objective To observe the effect of Shou-Hui Tongbian(SHTB)Capsules on postoperative ileus(POI)in mice,and explore its possible mechanism from the perspective of balance of gut microbiota.Methods POI model was es-tablished using classical small intestine interference technique.Thirty mice were randomly divided into Control group,POI group,positive control MSBL group,SHTB-L group,and SHTB-H group,with 6 mice in each group.The Control group and POI group were given physiological saline,the MSBL group was given Mosapride capsule solution(2.6 mg/kg),and the SHTB-L and SHTB-H groups were given SHTB capsule solution(0.619 g/kg,1.238 g/kg),qd by gavage for 2 consec-utive days.Ink propulsion method was used to detect the intestinal peristalsis ability of mice;H&E staining method to ob-serve the intestinal tissue structure;16S rRNA gene high-throughput sequencing technology to analyze the structure of in-testinal microbiota and identify the key bacteria.Results Compared with the Control group,the intestinal peristalsis abil-ity of POI group mice was significantly reduced,with an increase in neutrophils and lymphocytes in the small intestine mu-cosa accompanied by congestion,edema in tissue,a decrease in the total number of gut microbiota species,a significant de-crease in the relative abundance of beneficial bacteria Parvibater(t=3.340,P=0.008),and a significant increase in the relat-ive abundance of harmful bacteria Rikenellaceae_RC9_gut_group(U=2,P=0.009).The above changes had been allevi-ated to varying degrees after medication treatment.Compared with the POI group,the intestinal peristalsis ability of mice in SHTB-L,SHTB-H,and MSBL groups were significantly improved(all P＜0.010),the intestinal pathological changes such as inflammation,edema,and congestion were significantly improved;the total number of gut microbiota species in-creased,with a significant increase in Parvibacter relative abundance(U=5,P=0.041)and significant decrease in Rikenel-laceae_RC9_gut_group relative abundance(U=3,P=0.015).Conclusion Shou-Hui Tongbian Capsules can promote in-testinal peristalsis and improve intestinal pathological changes in POI mice by regulating the imbalance of key bacteria in the small intestine mucosa.
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