Changes in gut microbiota and metabolites in colorectal cancer patients based on 16S rRNA and non-targeted metabolome sequencing
Objective To observe the changes in intestinal flora and its metabolites of patients with colorectal cancer(CC)by using 16S rRNA and non-targeted metabolome sequencing analysis.Methods Ten CC samples and 6 normal control samples were collected for 16S rRNA sequencing.The composition,diversity and species differences of intestinal flora were analyzed,and the metabolic function of differential intestinal flora was predicted.Eight CC samples and 6 nor-mal control samples were subjected to LC-MS non-targeted metabolomics sequencing to analyze the metabolic changes and predict the function.Results The floral diversity in the CC group was not significantly different from that in the nor-mal control group(P＞0.05).In the CC group,the dominant phylum was Verrucomicrobia;the relative abundances of Sta-phylococcus,Psychrobacter,Lachnospiraceae_NK4A136_group,Clostridia_vadinBB60_group and CAG-56 increased(P＜0.05),while those of CAG-352,Runinococcus,Coprococcus and Eubacterium_eligens_group decreased(P＜0.05).KEGG enrichment analysis found that differential microflora plays important roles in pathways such as environmental in-formation processing,genetic information processing and metabolism.Non-targeted metabolomics sequencing detected significant differences in 367 metabolites in the two groups of samples,and the functions of differential metabolites was found to be associated with the metabolisms of caffeine,purine,amino sugar and nucleotide sugar and biotin.Conclusion The intestinal flora and metabolites of CC patients differ from those of healthy individuals,and there may be some association between CC and the intestinal flora and metabolic pathways.
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