Effects of oral probiotics on radiotherapy-related diarrhea and intestinal microbiota in patients with cervical cancer undergoing radical radiotherapy
Objective To evaluate the efficacy of oral probiotics in preventing radiotherapy-associated diarrhea(RD)in patients undergoing radical radiotherapy for cervical cancer and explore the impact of probiotics on the intestinal microbi-ota of these patients.Methods This study included 46 cervical cancer patients treated with radical radiotherapy and chemotherapy at our hospital from January 2020 to December 2022.The patients were randomly divided into an oral probi-otics group(OP group,n=23)and a non-oral probiotics group(NOP group,n=23).Fecal samples were collected from both groups before and after radiotherapy,and the intestinal microbiota was analyzed using 16S rDNA sequencing to assess the diversity of microbiota and differences between groups.Results The incidence of RD was 8.7％in the OP group vs 47.8％in the NOP group,indicating a statistically significant difference(P=0.009).Oral probiotics were able to increase the richness of intestinal flora in patients undergoing radiotherapy,but did not reverse the decline in α-diversity(P=0.012).No significant difference was observed in the p-diversity of intestinal microbiota before and after radiotherapy(P＞0.05).Species richness analysis revealed changes in the composition of intestinal microbiota at the phylum,family,genus and species levels after radiotherapy;notably,the relative abundance of Lactobacillus was higher before radiotherapy,but that of Moraxella was higher after radiotherapy among patients with diarrhea compared to those without diarrhea.Conclusion Oral probiotics during radical radiotherapy for cervical cancer can effectively prevent the occurrence of RD and influence the composition of intestinal microbiota,significantly reducing the relative abundance of anaerobic bacteria.
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