The impacts of high level squamous intraepithelial lesion and different subtypes of human papilloma virus infection on vaginal microbiota
Objective To observe the characteristics of vaginal microbiota in patients with high level squamous intrae-pithelial lesion(HSIL)and the impacts of different human papilloma virus(HPV)infection on vaginal microbiota.Methods The vaginal secretion samples from healthy women(control group,n=54)and HSIL patients(HSIL group,n=54)were collected.The HPV subtypes were detected using HPV kits.The composition,structure and characteristics of vaginal microbiota were detected with 16S rRNA gene high-throughput sequencing.Results The HPV positive rate was 88.9％in HSIL group vs 11.1％in control group.Compared with control group,the relative abundance of vaginal Lactoba-cillus in HSIL group decreased,while those of vaginal Gardnerella,Prevotella and Bifidobacterium increased,but with no statistically significant differences(all P＞0.05).Compared with the HPV Others group,the HPV CR group showed a sig-nificant decrease in the abundance of vaginal Atobobacter(t=2.067,P=0.043);those of vaginal Gardnerella,Streptococcus and Enterococcus decreased,while that of Bifidobacterium increased,but with no significant differences(all P＞0.05).Conclusion The HPV infection rate increases in HSIL patients.Different HPV subtype infection can impact the vaginal microbiota,suggesting that intervention in vaginal microbiota may have regulatory effect on cervical lesions.
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