Objective To observe the characteristics of vaginal microbiota in patients with high level squamous intrae-pithelial lesion(HSIL)and the impacts of different human papilloma virus(HPV)infection on vaginal microbiota.Methods The vaginal secretion samples from healthy women(control group,n=54)and HSIL patients(HSIL group,n=54)were collected.The HPV subtypes were detected using HPV kits.The composition,structure and characteristics of vaginal microbiota were detected with 16S rRNA gene high-throughput sequencing.Results The HPV positive rate was 88.9％in HSIL group vs 11.1％in control group.Compared with control group,the relative abundance of vaginal Lactoba-cillus in HSIL group decreased,while those of vaginal Gardnerella,Prevotella and Bifidobacterium increased,but with no statistically significant differences(all P＞0.05).Compared with the HPV Others group,the HPV CR group showed a sig-nificant decrease in the abundance of vaginal Atobobacter(t=2.067,P=0.043);those of vaginal Gardnerella,Streptococcus and Enterococcus decreased,while that of Bifidobacterium increased,but with no significant differences(all P＞0.05).Conclusion The HPV infection rate increases in HSIL patients.Different HPV subtype infection can impact the vaginal microbiota,suggesting that intervention in vaginal microbiota may have regulatory effect on cervical lesions.
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