Characteristics of pathogenic bacteria of pulmonary infection in ovarian cancer patients with post-chemotherapy bone marrow suppression and the correlation with expression of Nrf2 mRNA and TLR7 mRNA in peripheral blood mononuclear cells
Objective To observe the characteristics of pathogenic bacteria of pulmonary infection in ovarian cancer(OC)patients with post-chemotherapy bone marrow suppression(BMS),and the correlation with the expression of Nrf2 mRNA and TLR7 mRNA in peripheral blood mononuclear cells(PBMC).Methods A total of 30 OC patients with post-chemo BMS and pulmonary infection(pulmonary infection group),and 30 ones with post-chemo BMS but no pulmonary infection(non-pulmonary infection group)were included in the study.The characteristics of pathogenic bacteria in the pa-tients with pulmonary infection were observed.The expression levels of Nrf2 mRNA and TLR7 mRNA in peripheral blood mononuclear cells in the two groups after chemotherapy were detected.The relationship of Nrf2 mRNA and TLR7 mRNA with BMS,as well as the predictive values of Nrf2 mRNA and TLR7 mRNA for BMS complicated with pulmonary infec-tion were analyzed.Results A total of 30 pathogenic bacterial strains were detected,including 12(40.00％)strains of Gram positive bacteria and 18(60.00％)strains of Gram negative bacteria,with Pseudomonas aeruginosa being dominant pathogen(20.00％).The Nrf2 mRNA levels in the patients with grade 0-Ⅰ leukopenia and those with grade 0-Ⅰ neut-ropenia were significantly higher(all P＜0.05),while the TLR7 mRNA levels were significantly lower than those in the pa-tients with grade Ⅱ-Ⅲ leukopenia and those with grade Ⅱ-Ⅲ neutropenia,respectively(all P＜0.05).Leukopenia and neutropenia were negatively correlated with Nrf2 mRNA expressions,but positively correlated with TLR7 mRNA expres-sion(all P＜0.05).Nrf2 mRNA level in the pulmonary infection group was significantly lower,while TLR7 mRNA level was significantly higher than those in the non-pulmonary infection group,respectively(all P＜0.05).The sensitivity and specificity of Nrf2 mRNA,TLR7 mRNA,and the combination of both for predicting BMS complicated with pulmonary in-fection were 80.2％and 71.3％,83.5％and 70.9％,and 88.4％and 78.6％,respectively.Conclusion BMS is closely re-lated to Nrf2 mRNA and TLR7 mRNA in PBMC of OC patients after chemotherapy.BMS complicated with pulmonary in-fection is mainly caused by Gram-negative bacteria.PBMC Nrf2 mRNA level significant decreased and TLR7 mRNA level significantly increased in those patients.Combination of the two is of high predictive value for BMS complicated with pulmonary infection.
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