Effect of Qi-Ju-Dihuang Pills on intestinal mucosal microbiota of high-fat diet-induced obese mice
Objective To observe the effect of Qi-Ji-Dihuang Pills(QJDHP)intervention on intestinal mucosal microbi-ota in high-fat diet-induced obese mice using 16S rRNA gene sequencing,network pharmacology,and molecular docking techniques.Methods A total of 15 SPF male C57BL/6 mice were divided into three groups:normal(C group,n=5),mod-el(HF group,n=5)and QJDHP group(n=5).The HF and QJDHP groups underwent four months of high-fat diet,with the latter receiving QJDHP via gavage for one month.Sterile water was administered to the remaining groups.After the inter-vention,intestinal mucosal samples were collected for 16S rRNA gene sequencing analysis.The gutMGene database was used to predict diverse microbial metabolites.Network pharmacology was used to identify key QJDHP targets in obesity treatment,and molecular docking was used to assess their binding with microbial metabolites.Results NMDS and ANOSIM revealed significant inter-group differences(stress=0.057 7;R=0.411,P=0.005).Comparative species analysis highlighted an increased Firmicutes/Bacteroidetes(F/B)ratio in HF group vs.C group(t=3.649,P=0.006)and a decreased F/B ratio in QJDHP group vs.HF group.Cutibacterium and uncultured bacterium f Muribaculaceae were key genera dis-tinguishing HF group vs.C group and HF group vs.QJDHP group.Key obesity-related targets,including IL-6,were identi-fied for QJDHP intervention.Five metabolites displayed strong binding energies with both"IL-6"and"PPARG".Conclusion Qi-Ji-Dihuang Pills can reduce pathogenic bacteria,lower F/B ratio,regulate energy utilization,and de-crease fat storage.Benzoic acid and other gut microbiota metabolites may play a crucial role in Qi-Ji-Dihuang Pills's inter-vention on the intestinal microbiota in high-fat-induced obese mice.
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