The effect of Taohong Siwu Decoction on gut microbial diversity in mice with radiation-induced enteritis
Objective To observe the efficacy of Taohong Siwu Decoction(THSWD)on radiation-induced enteritis(RIE)in mice,and the probable mechanisms of action associated with gut microbiota.Methods Thirty six male SPF C57BL/6J mice were randomly divided into four groups:healthy control group(CON group),radiation enteritis model group(MODEL group),high-dose treatment group with THSWD(THSWD_H group),and low-dose treatment group with THSWD(THSWD_L group).Except for the CON group mice,which were only anesthetized,all the other groups of mice were subjected to 10 Gy of full abdominal radiation under anesthesia to establish the RIE models.Different concentrations of THSWD were orally administered at designated time points,while the CON and MODEL groups of mice were orally ad-ministered an equal volume of physiological saline.At the end of the experiment,the specimens of serum,colon tissue and colon contents were collected for H&E staining,ELISA detection,and 16S rRNA amplicon sequencing,respectively,to evaluate the effects of THSWD on inflammation status,vascular function,histopathological damage,and gut microbiota in mice.Results THSWD successfully reduced systemic inflammatory responses in RIE mice.Additionally,the serum levels of TNF-α,TGF-β,IL-6 and IL-22 in the intervention groups with varying dosages of THSWD were significantly de-creased,while that of IL-10 were significantly increased compared to the MODEL group respectively.Comparable out-comes were seen for the indicators of vascular function,VEGF and Ang Ⅰ.THSWD might ameliorate radiation-induced colon tissue damage in mice,according to the results of the H&E staining.Furthermore,THSWD intervention at varying concentrations could effectively improve the gut microbial disorder in mice with RIE.This was demonstrated by the gut microbiota of these mice at varying concentrations of THSWD,the alpha diversity of which was higher than that in the MODEL group,and beta diversity was more similar to that in the CON group.Conclusion THSWD has a good thera-peutic effect on mice with RIE,which can significantly improve gut microbial disorder and vascular function damage caused by radiation exposure.
Radiation therapyHeat toxic damage to collateralsRadiation enteritisTaohong Siwu decoctionGut micro-biota
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