Differences in oral microbiota of patients with dental caries at different ages:insights from low-abundance taxa
Objective To understand the differences in the composition of oral microbiota across age gradient for tailor-ing treatment strategies for patients with dental caries.Methods Supragingival dental plaque samples and saliva samples(n=24)were collected from a wide age range(children group,youth group and elderly group)of patients with caries.High-throughput sequencing of 16S rRNA gene was employed to explore the relationship between age and oral microbiota in pa-tients with dental caries.Results The α-diversity of oral microbiota was the highest in the elderly group(P＜0.05),while the β-diversity in the youth group displayed the greatest variation.Meanwhile,the differences in the structure and variation of oral microbiota communities among cary patients of varying ages were analysed through the co-occurrence network as well as shared and endemic species in cary patients of different age groups.Concretely speaking,Capnocytophaga,Tanner-ella,Centipeda,Cardiobacterium and Aggregatibacter,as well as the endemic genera Holdemanella,Tyzzerella,Lachnos-piraceae,Catenisphaera and Sarcina played an important role in bacterial community in children group.In the co-occur-rence network of middle-aged patients,the key genera Dialister,Family_ⅩⅢ_UCG-001 and Johnsonella,and the endemic genera Anaerovibrio,Candidatus_Tammella and MND1 of the family Nitrosomonadaceae played an important role in the community of the youth group.The key genera in the co-occurrence network of elderly group were norank_f_Leptotrichi-aceae of the family Ciliobacteriaceae,Eubacterium nodatum_group,Roseburia,Streptococcus and Microbacterium.All of these genera except Streptococcus and Aggregratibacter had relative abundances of less than 0.1％.Conclusion The im-portance of low-abundance taxa in shaping the oral microbiota and provide insights into the age-related differences in pa-tients with dental caries.
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