Changes of oral flora,gingival crevicular fluid inflammatory factors and immune function before and after treatment of patients with erosive ulcerative oral mucosal disease
Objective To explore the changes and relationship among oral flora,gingival crevicular fluid inflammatory factors and immune function before and after treatment of patients with erosive ulcerative oral mucosal disease.Methods A total of 80 patients with erosive ulcerative oral mucosa treated in our hospital from October 2020 to October 2023 were selected as the study objects.All patients were treated with Triamcinolonede combined with recombinant hu-man epidermal growth factor.The changes of oral microflora,gingival sulcus fluid inflammatory factors[tumor necrosis factor-α(TNF-α),C-reactive protein(CRP),interleukin-6(IL-6)],and immune function[CD4+,CD8+,NK cells]before and after treatment were compared.Pearson correlation analysis was used to analyze the relationship between oral flora and gingival crevicular fluid inflammatory factors and immune function in patients with erosive ulcerative oral mucosa.Results Compared with before treatment,the levels of TNF-α,CRP and IL-6 were decreased after treatment(all P＜0.05).Compared with before treatment,CD4+cells increased,CD8+and NK cells decreased after treatment(all P＜0.05).At the genus level,there was a statistically significant difference in the abundance of Streptococcus,Neisseria,and Veillonella before and after treatment(all P＜0.05).At the phylum level,after treatment,the abundance of Firmicutes and Actinobacteria significantly increased,while the abundance of Bacteroidetes,Proteobacteria,and Fusobacteria signific-antly decreased(all P＜0.05).The Alpha diversity analysis showed that the Chaol index,Observed species index,Shan-non index and Simpson index were all higher after treatment than before treatment(all P＜0.05).Pearson correlation ana-lysis showed that Streptococcus,Neisseria,Veillonella,Firmicutes,Actinobacteria,Chaol index,Observed species index in patients with erosive ulcerative oral mucosa were negatively correlated with TNF-α,CRP,IL-6,CD8+ cells and NK cells,but positively correlated with CD4+cells(all P＜0.05);Bacteroidetes,Proteobacteria and Fusobacteria were positively cor-related with TNF-α,CRP,IL-6,CD8+ cells and NK cells,but negatively correlated with CD4+cells(all P＜0.05).Conclusion After treatment,the structure of oral flora in patients with erosive ulcerative oral mucosa is changed,the level of gingival crevicular fluid inflammatory factors is decreased,and the immune function is enhanced.The changes of oral flora in patients with erosive ulcerative oral mucosa may be related to the inflammatory factors and immune function of gingival crevicular fluid.
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