The changes of intestinal microbiota and intestinal barrier function in patients with pulmonary infection after spinal surgery
Objective To explore the changes of intestinal microbiota and intestinal barrier function in patients with pul-monary infection after spinal surgery,and discuss the correlations,providing a reference for the treatment.Methods One hundred and fifty patients who underwent spinal surgery in our hospital from January 2020 to December 2022 were selec-ted,and assigned into infection group(n=53)and non-infection group(n=97)according to the presence or absence of post-operative pulmonary infection.The disturbance of intestinal microbiota and bacterial count were detected,and the serum levels of diamine oxidase and D-lactate were measured using enzyme-linked immunosorbent assay.The relative abund-ance of gut microbiota was analyzed through 16S rRNA gene sequencing.Pearson correlation analysis was conducted to verify the correlation between gut microbiota,barrier function indicators,and pulmonary infections after spinal surgery.Results There were 22 cases with normal gut microbiota in infection group and 76 cases in non-infection group.The dis-turbance rate of gut microbiota was 58.49％in infection group,which was higher than the 21.65％in non-infection group(all P＜0.05).The counts of Lactobacillus,Bifidobacterium and Bacteroides in infection group were lower,while those of Enterococcus and Peptostreptococcus were higher than those in non-infection group,respectively(all P＜0.05).The serum levels of diamine oxidase and D-lactate in infection group were higher than those in non-infection group,respectively(all P＜0.05).A total of 342 bacterial genera were found in the microbiota samples of 150 patients undergoing spinal surgery.The relative abundances of Peptostreptococcus and Enterococcus in the infection group were higher,while those of Bac-teroides,Bifidobacterium and Lactobacillus were lower than those in the non-infection group,respectively(all P＜0.05).Pearson correlation analysis denoted that the counts of Lactobacillus,Bifidobacterium and Bacteroides were negatively correlated with postoperative pulmonary infection rate,while those of Enterococcus,Peptostreptococcus and serum levels of diamine oxidase and D-lactic acid were positively correlated with postoperative pulmonary infection rate(all P＜0.05).Conclusion Patients with pulmonary infection after spinal surgery suffer disturbance of intestinal microbiota and de-creased intestinal barrier function,and the two abnormal changes are related to the severity of pulmonary infection.
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