Mechanism of action of Bifidobacteria on PI3K/AKT signaling pathway in SW480 strain of colon cancer cells
Objective To explore the effect and mechanism of whole peptidoglycan(WPG)in epithelial stromal trans-formation(EMT)in the human colon cancer cell line SW480.Methods Three groups were designed:control group(SW480 colon cancer cells),EMT group(TGF-β1 induction group)and EMT+WPG group(WPG was added after TGF-β1 induction).The proliferative capacity of the cells in each group was determined with CCK-8 assay;the scratch experiment to detect the migration ability of the cells;and Transwell to determine the invasion ability of each group of cells.The ex-pressions of p-PI3K/PI3K,p-AKT/AKT,Vimentin,and E-cadherin proteins were detected using Western Blot;apoptosis of the cells was determined using flow cytometry.Results TGF-β1 induced EMT of SW480 in human colon cancer cells(P＜0.050 0).Higher proliferation(t=0.795 3,P=0.449 4),migration(t=1.289 0,P=0.267 0)and invasion(t=7.583 0,P＜0.000 1)were seen in TGF-β1 induced EMT group than in controls,while the proliferation(t=3.665 0,P=0.006 4),mi-gration(t=5.121 0,P=0.006 9)and invasion(t=7.711 0,P=0.000 1)in EMT+WPG group were lower than in TGF-β1-in-duced EMT group.The expression of p-PI3K/PI3K(t=5.785 0,P=0.004 4),p-AKT/AKT(t=1.128,P=0.007 1)and Vi-mentin(t=3.339 0,P=0.002 4)in TGF-β1 induced EMT groups increased compared with control group,while that of E-cadherin reduced(t=4.854 0,P=0.008 3);the difference was statistically significant.The expressions of p-PI3K/PI3K(t=4.131 0,P=0.000 2),p-AKT/AKT(t=3.721 0,P=0.033 7)and Vimentin(t=1.756 0,P=0.091 0)were lower in EMT+WPG group than in TGF-β1 induced EMT group,while that of E-cadherin increased(t=1.645 0,P=0.115 0),and cell apoptosis increased(t=13.400 0,P=0.000 2);the difference was statistically significant.Conclusion WPG inhibited the epithelial stromal transformation of colon cancer cell line SW480 and then inhibited the invasion and metastasis of colon cancer cells through regulating PI3K/AKT signaling pathway.
Whole peptidoglycanSW480 cell lineEpithelial stromal transitionInvasion and metastasis
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