The effects of long-term moderate alcohol consumption on physiological indicators and gut microbiota in rats
Objective To observe the effects of long-term moderate alcohol consumption on blood lipids,liver and gut microbiota in rats.Methods Forty male SD rats were randomly divided into the control group,Baijiu(liquor)group,wine group,yellow rice wine group and ethanol group,and gavaged with distilled water,Baijiu,wine,yellow rice wine and eth-anol respectively for 90 days.A fully automated biochemical analyzer was used to detect the concentrations of serum cho-lesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),and blood glucose levels.HE staining was used to observe liver pathological changes,and 16S rRNA genes sequencing was used to analyze changes of gut microbiota in each group.Results Moderate alcohol consumption did not increase the body weight and organ mass of rats during the experiment,and did not significantly increase the serum blood glucose,TG,and TC levels.Pathological analysis showed that moderate alcohol consumption caused structural abnormalities in liver tis-sue and steatosis of liver cells.Long term moderate alcohol consumption increased the relative abundances of Clostridium_Ⅺ Ⅴ a,Oscillibacter.Flavonifractor,Roseburia(belonged to Bacillota)and those of Prevotella and Bac-teroides(belonged to Bacteroidota),while decreased the relative abundances of Lactobacillus,Eubacterium,and Rumino-coccus.Conclusion Long term moderate alcohol consumption can cause mild liver inflammation,decrease the relative abundance of beneficial bacteria in gut microbiota,and increase those of opportunistic pathogenic bacteria,thereby affect-ing the body's health.
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