Changes of intestinal flora,OPG/RANKL/RANK pathway in kidney-yang deficiency COPD combined with osteoporosis and therapeutic effect of probiotics in rats
Objective To observe the changes of intestinal flora and osteoprotegerin(OPG)/receptor activator for nucle-ar factor-κB ligand(RANKL)/receptor activator for nuclear factor-κB(RANK)pathway,and the therapeutic effect of probi-otics in rats with kidney-yang deficiency chronic obstructive pulmonary disease(COPD)complicated with osteoporosis.Methods Male SD rats were randomly divided into control group,model group and probiotic group,with 8 rats in each group.Kidney-yang deficiency COPD complicated with osteoporosis rat models were established in model group and pro-biotic group.The model group was given normal saline while the probiotic group was given a probiotic[0.9×109 CFU/(g·kg·d)]for 28 days.Pulmonary function forced vital capacity(FVC),forced expiratory volume 0.2 second(Fev 0.2),bone mineral density(BMD)of femur,serum levels of interferon-γ(IFN-γ),interleukin-4(IL-4),alkaline phosphata-se(ALP),procollagen Ⅰ N-terminal propeptide(PINP),tartrate resistant acid phosphatase(TRAP)and β C-terminal cross-linked telopeptides of type Ⅰ collagen(β-CTX),the mRNA expression levels of OPG,RANK and RANKL in lung tissue and bone tissue were measured.Results The levels of FVC,Fev 0.2,BMD,serum ALP and PINP contents,Ace index,Chaol index,Shannon index of intestinal flora richness,OPG mRNA expression in lung tissue and bone tissue in model group were lower,while the proportion of serum IFN-γ/IL-4,contents of IFN-γ,TRAP,β-CTX,mRNA expression levels of RANK and RANKL in lung and bone tissues were higher than those in control group,respectively(all P＜0.05).The levels of FVC,Fev 0.2,BMD,serum ALP and PINP contents,Ace index,Chaol index,Shannon index of intestinal flora richness,OPG mRNA expression in lung tissue and bone tissue in probiotic group were higher,while the proportion of ser-um IFN-γ/IL-4,contents of IFN-γ,TRAP,β-CTX,mRNA expression levels of RANK and RANKL in lung and bone tis-sues were lower than those in the model group,respectively(all P＜0.05).Conclusion Rats with Kidney-yang defi-ciency COPD combined with osteoporosis have intestinal flora imbalance and abnormal OPG/RANKL/RANK pathway.Supplementation of probiotics can significantly correct intestinal flora imbalance and abnormal OPG/RANKL/RANK path-way,and improve lung function and bone mineral density.
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