Changes of gut microflora in patients with atrial fibrillation and its correlation with recurrence after catheter ablation
Objective Using 16S rDNA sequencing technology to detect the changes in diversity and abundance of in-testinal bacteria in patients with atrial fibrillation(AF),observe the differences in fecal bacteria between non-recurrence and recurrence groups after catheter ablation,and analyze their correlation.Methods In a prospective cohort study,53 pa-tients with AF admitted from January to May 2021 were included as the AF group according to the criteria and underwent catheter ablation,then subdivided into non-recurrence group(47 cases)and recurrence group(6 cases)according to wheth-er AF recurred after the procedure.29 healthy individuals with matching gender and age who accepted physical examina-tion during the same period were selected as the healthy control group.All the subjects were tested for intestinal flora us-ing the 16S rDNA assay.Results The alpha diversity of intestinal flora was lower in the AF group compared to the con-trol group.At the phylum level and class level,there were no significant difference in community structure between the two groups(all P＞0.05).The relative abundances of Dinococcus-Thermophilus(Q=0.020,P=0.001),Thermophilus(Q=0.023,P＜0.001),Phylum WSP-1(Q=0.025,P＜0.001),Ktedonobacteria(Q=0.028,P＜0.001),Thermomicrobia(Q=0.037,P＜0.001),Bacillales(Q=0.018,P＜0.001),Tepidisphaerales(Q=0.025,P＜0.001),Phylum JG30-KF-CM45(Q=0.029,P＜0.001),Phylum JG30-KF-AS9(Q=0.032,P＜0.001),Family DA111(Q=0.039,P＜0.001)and Family BIrii41(Q=0.045,P＜0.001)were lower in the AF group than in the control group.At the genus level,there were no signifiicant differences between the two groups.In the AF group,the relative abundances of Proteobacteria,Spirochaetae,Betaproteo-bacteria,Epsilonproteobacteria,Spirochaetes,JG30_KF_AS9,Burkholderiales,Campylobacterales,Spirochaetales,Entero-coccaceae and Alcaligenaceae increased in the recurrence group,and those of Neisseriales,Neisseriaceae,Christensenel-laceae and Rikenellaceae increased in the non-recurrence group.In the two subgroups,the highest LDA scores were found in Proteobacteria,with LDA scores＞4.Conclusion The intestinal flora diversity reduced in the AF group compared with the healthy control group,and there was no significant difference in community structure.Patients in the recurrence group had disordered gut flora compared to the non-recurrence group,which may be a potential predictor for recurrence of AF after catheter ablation.
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