Levels of serum suPAR and GRP78 in patients with colon cancer and their correla-tion with intestinal flora dysbiosis
Objective To observe the levels of soluble urokinase plasminogen activator receptor(suPAR)and glucose regulated protein 78(GRP78)in sera of patients with colon cancer(CC)and their correlation with intestinal flora dysbiosis.Methods A total of 116 patients with CC in our hospital from May 2020 to May 2023 were selected as the CC group,with 114 healthy subjects in the same period selected as the control group.Serum levels of suPAR and GRP78 were detec-ted with ELISA;fresh stool was collected,and intestinal flora was measured.According to the distribution of intestinal flora,the CC groups were divided into normal flora group(n=41),dysfunctional flora group Ⅰ(n=30),dysfunctional flora group Ⅱ(n=24)and dysfunctional flora group Ⅲ(n=21).Serum levels of suPAR and GRP78 and the counts of enterococcus,bifidobacterium and lactobacillus were compared among the four groups,and the correlation of serum levels of suPAR and GRP78 levels with intestinal flora was evaluated.Results The serum levels of suPAR and GRP78 in the CC group were significantly higher than those in the control group(P＜0.001);the more severe the intestinal disorder was,the higher the serum levels of suPAR and GRP78 were(P＜0.001).The count of enterococcus in the CC group was more(P＜0.001),while those of bifidobacterium and lactobacillus were less than those in the control group,respectively(P＜0.001);the more severe the intestinal disorder was,the less the counts of bifidobacterium and lactobacillus and the more that of entero-coccus were(P＜0.001).Pearson correlation analysis showed that the levels of suPAR(r=0.830,P＜0.001)and GRP78(r=0.766,P＜0.001)were positively correlated with the count of enterococcus,and negatively correlated with those of bi-fidobacterium(r=-0.799,P＜0.001;r=-0.780,P＜0.001)and lactobacillus(r=-0.792,P＜0.001;r=-0.712,P＜0.001).Con-clusion The serum suPAR and GRP78 increased in patients with colon cancer,which may be related to the intestinal flora and dysbiosis.
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