Evaluation of probiotic bioactivity of epimedium total flavonoids based on Bifidobacterium longum
Objective To observe the regulatory effects of total flavonoids of epimedium(TFE)on the growth and meta-bolism of Bifidobacterium longum and the anti-inflammatory activity of Bifidobacterium longum fermentation broth.Methods TFE at concentrations of 0.5％,1.0％and 2.0％was added to a glucose-deficient MRS medium as the culture base.With the media containing 2.0％glucose and 2.0％fructooligosaccharides as the controls,the colony forming unit(CFU),OD value and other indicators of Bifidobacterium longum were measured after fermentation to assess the impact of TFE on the growth of Bifidobacterium longum and the in vitro antioxidant activity of the fermentation broth was measured using l,l-diphenyl-2-picrylhydrazyl(DPPH).RAW264.7 cells induced by LPS were used as an inflammation model to evaluate the anti-inflammatory activity of the fermentation broth.The levels of relevant inflammatory factors before and after fermentation were assessed to determine the anti-inflammatory effect.Results TFE significantly promoted the pro-liferation of Bifidobacterium longum.The growth promoting rates of different concentrations of TFE(5 mg/mL,0.5 mg/mL and 0.05 mg/mL)compared to the control group were 59％,23％,and 20％,respectively.The 24-hour growth curve also in-dicated the proliferative effect of TFE.Research on gastrointestinal tolerance found that adding 2％TFE to the growth en-vironment of Bifidobacterium longum can improve the stability of the strain in 0.3％bile salt(t=6.008,P＜0.01),artificial gastric juice(t=4.371,P＜0.05),and pH3 acidic environment(t=9.645,P＜0.001),and increased the growth concentration and in vitro antioxidant capacity of the strains in culture media containing chlortetracycline,clindamycin,kanamycin,met-ronidazole,and vancomycin(t=15.141,P＜0.001).In addition,adding TFE to the fermentation broth of Bifidobacterium longum reduced the levels of pro-inflammatory factors TNF-α(t=4.655,P＜0.01),IL-1β(t=7.775,P＜0.01),and IL-6(t=7.775,P＜0.01),thus improved LPS induced inflammatory response.Conclusion TFE can promote the growth and strain stability of Bifidobacterium longum,and enhance its anti-inflammatory activity,which has the potential to become a new source of probiotics for traditional Chinese medicine.
Total flavones of epimediumBifidobacterium longumPrebioticsAnti-inflammatory
NETL
NSTL
万方数据
