The effects of Limosilactobacillus WMU003 on cognitive impairment and neuronal damage in an Alzheimer's disease model mouse via the gut-brain axis
Objective To investigate the ameliorative effects of Limosilactobacillus WMU003 on cognitive impairment and neuronal damage in APP/PS1 mice via regulating gut microbiota-gut-brain axis.Methods Male APPswe/PSEN1dE9 transgenic mouse were treated with Limosilactobacillus WMU003.Cognitive function was assessed using the nesting test and open field test.The impact of Limosilactobacillus WMU003 on Alzheimer's disease(AD)histopathology changes were analyzed using Nissl staining and Congo red staining,and changes in gut microbiota were evaluated using 16S rRNA gene sequencing.Results Compared to the APP/PS1 group,the APP/PS1+Lf treatment group showed improved scores of nest-ing function,decreased time spent in the open field test,increased locomotor activity,and higher average speed.There was an increase in the number of Nissl bodies in the cerebral cortex and hippocampal region,a decrease in Aβ deposition in the brain,and significant changes in alpha diversity of gut microbiota.At the family level,there was a significant increase in Saccharimonadaceae abundance in the APP/PS1 group,while Muribaculaceae and Rikenellaceae abundance significantly increased in the APP/PS1+Lf treatment group.At the genus level,Candidatus_Saccharimonas,Gordonibacter and Eubac-terium_xylanophilum_group abundance significantly increased in the APP/PS1 group,whereas Dubosiella,Prevotellaceae_UCG-001,Faecalibaculum and Marvinbryantia abundance significantly increased in the APP/PS1+Lf treatment group.Conclusion Limosilactobacillus WMU003 ameliorated cognitive impairment and neuronal damage in AD by regulating the gut microbiota-gut-brain axis.
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