The effect of probiotics Nagqu 4580 on sleep disorder in chronic restraint stress mice
Objective To observe the ameliorating effect of postbiotics Nagqu 4580 on sleep disorders in chronic re-straint stress(CRS)mice.Methods 225 Male C57BL/6J mice,aged 6 to 8 weeks and weighed 18-22 g,were randomly devided into control group,CRS model group and three observation groups(low-,medium-and high-dose groups)and 75 mice in each group.The modeling of CRS was carried out during continuous 21 days in CRS model group and observation groups.The mice in observation groups were gavaged with low-[1 g/(kg·bw·d)],medium-[2 g/(kg·bw·d)]and high-dose[3 g/(kg·bw·d)]of Nagqu 4580 while the other groups were gavaged normal saline,respectively,for 30 days.The sucrose preference test,direct sleep test,sodium barbital-induced sleep latency test,sub-threshold sodium pentobarbital dose-in-duced sleep test and sodium pentobarbital-induced sleep duration test were performed in all groups on Day 30 after gavage.The thymuses,livers,spleens of mice were collected and the organ coefficients were calculated after the tests.Results Compared with control group,the weight and sucrose preference index of mice in CRS model group and obser-vation groups decreased significantly after modeling of CRS(t=6.444,P=0.001;t=2.229,P=0.029).Compared with CRS model group,the weight of mice in low-dose group increased significantly(t=2.347,P=0.022)and the sucrose preference index in observation groups raised.Each group of mice didn't fall asleep in the direct sleep test.Compared with CRS mod-el group,the sleep latency in high-dose group increased significantly in the sodium barbital-induced sleep latency test(t=-2.575,P=0.018).The number and the rate of asleep mice in the sub-threshold sodium pentobarbital dose-induced sleep test increased significantly in medium-dose group(P=0.025).Conclusion Relief of axiety and depression and alleviation of sleep disorders in CRS-induced mice are the main functions of probitoics Nagqu 4580 rather than direct hypnotizing.Compared with other doses,the medium dose is better.
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