The differences in gut microbiota for colorectal cancer patients with Liver and kidney Yin deficiency syndrome and Qi and blood deficiency syndrome
Objective To explore the differences in the structure of intestinal flora in deficiency syndrome colorectal can-cer(CRC)patients with liver and kidney Yin deficiency syndrome(LKYDS)and Qi and blood deficiency syndrome(QBDS),and provide reference for the differentiation and classification of CRC.Methods Fecal samples from patients with primary colorectal adenocarcinoma were collected and classified according to TCM syndrome differentiation into LKYDS group and QBDS group.A total of 32 patients were enrolled.There were 19 cases in the LKYDS group and 13 cases in the QBDS group.16S rDNA amplification sequencing method was used to identify the diversity and abundance of intestinal flora in the two groups,and the species differences between the groups were analyzed.Results There were no significant differences in Shannon,Simpson,and Chao1 indexes between the 2 groups(all P＞0.05).The ACE index in the QBDS group(924.78±350.46 vs 885.82±558.62,P=0.032)was significantly higher than that in the LKYDS group.UP-GMA sample clustering tree,PCoA and Anosim(R=-0.194 3,P=0.919)analyses showed that there was no significant dif-ference between the 2 groups(all P＞0.05).At the phylum level,the intestinal flora of patients in both groups were domin-ated by Firmicutes,Bacteroidota,Proteobacteria,Actinobacteriota and Verrucomicrobiota.LEfSe analysis showed that the QBDS group had 13 biomarker flora;the iconic flora at the genus level were Lactobacillus,Eggerthella,Ruminococcus and Clostridium_innocuum_group,Christensenellaceae_R_7_group and UCG_005.LKYDS group has 7 biomarker flora,and the iconic flora at the genus level were Megamonas,Alloprevotella,Peptostreptococcus and Prevotella_9(all P＜0.05).Conclusion Although there are certain similarities in the LKYDS and QBDS in CRC,their differential flora may help to distinguish clinical TCM syndromes.
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